TSH assay using dried blood samples: a screening method for congenital hypothyroidism.

Capillary blood samples from three hundred healthy, full-term newborn within 48 and 120 hours of delivery, were collected on filter paper for analysis of thyroid-stimulating hormone (TSH). Blood was collected by pricking the heel of the newborn and spotted and dried on filter paper cards. The samples were analysed by immunoradiometric assay (IRMA) to determine the TSH level. Some fifteen percent of a total 1928 deliveries at two hospitals during a period of one year were included in the study. Male:female ratio was 1.1:1. There was no maternal history of thyroid disease. None of the babies had any clinical feature of hypothyroidism. On analysis, TSH level ranged from 0.6-19.3 microU/ml with a mean (SD) of 7.19 (4.21) microU/ml. TSH assay from dried filter paper blood spot is technically possible and is a cost-effective and reliable method for a screening programme. This is the first time in Bangladesh that this method has been successfully carried out.

Relationship of prolactin and gonadotrophic hormones in regulation of the ovarian function.

Circulating concentration of the LH, FSH, prolactin (PRL), oestradiol-17 beta (E2) and progesterone (P) in serum were investigated in women with normal cycles and proven fertility, with irregular cycles and infertility and with primary or secondary amenorrhea in order to understand the relationship of the prolactin and gonadotrophic hormens in regulation of the ovarian function. The results indicate that hyperprolactinemia inhibits the pituitary to release the midcycle LH surge in response to the positive feedback action of the elevated oestrogen at the midcycle. The elevated LH and FSH levels found in the cases of the secondary amenorrhea is the indication of the absence of the steroid negative feedback due to the ovarian failure. Prolactin does not seem to play a role on the manifestation of either primary or secondary amenorrhea.

Metabolic and hormonal profile of the offsprings of conjugal diabetics.

Oral glucose tolerance response to blood glucose, serum immunoreactive insulin (IRI) and plasma free fatty acid (FFA) levels were studied on 159 offsprings of both parent diabetics (connubials). Fasting serum cholesterol, triglyceride, phospholipid and total lipids were also measured in these subjects. We detected 6 diabetics out of 159 connubials at the time of our study. FFA level in 6 diabetic connubials were higher all time intervals than 153 non-diabetics connubials. Fasting and one hour post glucose response were less in 6 diabetic connubial but two hours post glucose IRI response to both 6 diabetic connubials and 153 non-diabetic connubials were same indicating a delayed insulin secretion in response to oral glucose level in 6 diabetic connubials.